In this paper we present an

2018-10-20

In this paper, we present an all-in-one platform, Pulse, which uses video microscopy and image-analysis algorithms (Maddah and Loewke, 2014) to automatically capture and quantify the beating patterns of cardiomyocytes. Our technique generates a beating signal that corresponds to the biomechanical co

The trend for increased differentiation in the field is

2018-10-20

The trend for increased differentiation in the field is paralleled by a large and increasing proportion of papers in which both cell types are being used. To analyze the validity of the “gold standard” argument that is frequently used to justify the continued use of hESCs in research, we analyzed th

In Stevens and colleagues found penta snRNP which contains a

2018-10-20

In 2002, Stevens and colleagues found penta-snRNP, which contains all five spliceosomal snRNAs and over 60 pre-mRNA splicing factors, in yeast. They proposed that penta-snRNP associates prior to pre-mRNA substrate binding rather than with the pre-mRNA based on the results of a stepwise addition of d

In this work we developed

2018-10-20

In this work, we developed multiple tools to interrogate this autocrine and paracrine signaling loop involving α-5 laminin production by hPSCs. The use of the inducible short hairpin lines allowed us to control the dynamics of α-5 laminin production in a reversible manner. However, the interpretatio

Here we study the impact of the

2018-10-20

Here we study the impact of the common GBA-N370S mutation on the phenotype of patient-specific dopaminergic neuronal cultures differentiated from iPSC lines derived from patients with PD and identify deficits in protein homeostasis. Our results suggest that the heterozygous GBA-N370S mutation leads

Nishi et al a b have developed a method of

2018-10-20

Nishi et al. (2014a, 2014b) have developed a method of inducing cancer stem-like ceramidase (CSCs) through the reprogramming and partial differentiation of the immortalized but otherwise normal MCF10A human mammary epithelial cell line. To experimentally test our computational model of oncometaboli

Generation of new cells during kidney repair has

2018-10-20

Generation of new cells during kidney repair has long been assumed to result from proliferation of surviving terminally differentiated cells. However, much of the modern evidence has used lineage tracing such as Six2 (Humphreys et al., 2008) or a terminally differentiated marker (such as the proxima

The pituitary gland is considered the master

2018-10-20

The pituitary gland is considered the master gland of hormone function. Hypopituitarism is a disease condition with insufficient or absent function of the pituitary gland. Pituitary tumors are the most common cause but many other triggers can induce pituitary dysfunction including inborn genetic def

Among the epigenetic aberrations of iPSCs

2018-10-20

Among the epigenetic aberrations of iPSCs, DNA methylation is of particular importance. Previous studies showed that unique de novo differentially methylated (DMR) or hydroxymethylated regions (hDMR) are present in iPSCs compared with hESCs (Lister et al., 2011; Wang et al., 2013). Furthermore, the

Whether LIF contributes to differentiation inhibition in rab

2018-10-20

Whether LIF contributes to differentiation inhibition in rabbit PSCs is unclear. LIF and FGF2 cooperatively support self-renewal in ESCs derived from parthenogenotes and propagated in feeder-free conditions, but the effect of LIF is modest (Hsieh et al., 2011). In this study, AKSL and AKSgff cell li

Pitavastatin a hydroxy methyl glutaryl coenzyme A HmG

2018-10-20

Pitavastatin, a 3-hydroxy-3-methyl-glutaryl-coenzyme A (HmG-CoA) reductase inhibitor, was cytotoxic only to undifferentiated NPCs, whereas another HmG-CoA reductase inhibitor, cilastatin, had no effect. Similarly, 5-FU was substantially more toxic to undifferentiated cultures and resulted in nearly

For the photoreceptors to function

2018-10-20

For the photoreceptors to function, recycling of visual pigments by isomerization and oxidation, from all-trans retinol to 11-cis retinol, and to 11-cis retinal, is essential (Parker and Crouch, 2010). The first essential step of isomerization to form 11-cis retinol is catalyzed by RPE65 in RPE for

Hello world!

2018-07-29