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Immunofluorescence Cells were fixed with paraformaldehyde PF
2020-03-26
Immunofluorescence. Cells were fixed with 4% paraformaldehyde (PFA) in PBS and extracted by 0.5% Triton X-100-PBS, or fixed in cold methanol. The cells were immunostained with mouse monoclonal Ciclopirox ethanolamine sale against α-tubulin (Sigma), γ-tubulin (Sigma), Ran (Upstate), rabbit polyclonal
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The use of AACSB accreditation as an
2020-03-26
The use of AACSB accreditation as an indicator of expected performance on the CPA exam has been examined by prior studies (e.g. Barilla et al., 2008, Boone et al., 2006, Grant et al., 2002, Howell and Heshizer, 2006, Lindsay and Campbell, 2003, Marts et al., 1998). While these studies are based on d
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br Acknowledgments We thank the Lapaha Community and
2020-03-26
Acknowledgments We thank the Lapaha Community and Nobles (His Serene Highness Prince Kalaniuvalu Fotofili, Her Serene Highness Princess Marcella Taumoepeau Tupou Kalaniuvalu Fotofili and Her Royal Highness Mele Siu\'ilikutapu Kalaniuvalu Fotofili) and the Ministry of Internal Affairs (Government
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To characterize Ea and S
2020-03-26
To characterize Ea and γ·S, DC/AC electrical parameters will be determined from MIL standard measurements in a temperature range of −55 to +200°C. It is therefore necessary also to characterize the junction temperatures and hot points by infra-red or electrical method. The accelerated aging, represe
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This study demonstrates that GUL affects multiple
2020-03-26
This study demonstrates that GUL-1 affects multiple pathways/processes. As GUL-1 is likely to function as an RNA-binding protein, it is conceivable that the mRNAs involved are bound to GUL-1 as part of the regulatory function of the protein. In fact, in S. cerevisiae Ssd1 was shown to bind multiple
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br Introduction To detect exposure
2020-03-26
Introduction To detect exposure to and effects of neuroactive pesticides in aquatic organisms, one widely used biomarker is cholinesterase (ChE) activity, which, among other possible functions, catalyzes the hydrolysis of the neurotransmitter 98014 (ACh) in the cholinergic synapses of the centra
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br Halogenases Enzymatic C H activation
2020-03-26
Halogenases Enzymatic C–H activation leading to halogenation is another emerging area in biocatalysis [55,56]. Incorporation of halogen atoms during medicinal chemistry eff ;orts is a well-established practice, presenting an effective means to control the molecule’s bioactivity and physicochemical
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OxLDL down regulates eNOS and
2020-03-26
OxLDL down-regulates eNOS and up-regulates iNOS, thereby augmenting the formation of NO and protein S-nitrosylation in human endothelial Cy5 Firefly Luciferase mRNA [26]. Importantly, iNOS-mediated S-nitrosylation plays an increasingly significant role in cardiovascular diseases [34]. For example,
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br Other factors regulating DNA PKcs Recent work
2020-03-26
Other factors regulating DNA-PKcs Recent work in the field has also focused on factors that regulate NHEJ and NHEJ factors, in particular proteins that modulate DNA-PKcs. In this section we will highlight a number of factors which have been found to regulate DNA-PKcs. DNA-PKcs phosphorylation at
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br In half a century after
2020-03-26
In 2015–2016, half a century after its first biochemical characterization as a template-independent polymerase, it was shown that TdT can, firstly, assemble a DNA synapsis by itself, optimally with one micro-homology base-pair between strands [] and secondly, perform a template-dependent nucleotid
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br Sphingosine kinase One of the enzymes
2020-03-26
Sphingosine kinase One of the enzymes closely related to DGKs are the sphingosine kinases (SKs). These enzymes catalyze the conversion of sphingosine (Sph) to sphingosine-1-phosphate (S1P). SKs and DGKs are closely related lipid kinase in term of basic enzymology and the mechanism of regulation a
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Substituents at the C position An oxygen containing substitu
2020-03-26
Substituents at the C11 position — An oxygen-containing substituent at the C11 position also affects the catalytic activity of Δ1-KSTDs. The Δ1-KSTDs from the Gram-positive bacteria R. equi [29], N. simplex ATCC 6946 and IFO 12069 [48,49,52], and R. rhodochrous IFO 3338 [27] were able to 1(2)-dehydr
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br Acknowledgment We thank Professor Yuh Chang Sun
2020-03-26
Acknowledgment We thank Professor Yuh-Chang Sun for providing helpful advice and the Ministry of Science and Technology of the Republic of China for financial support (grants MOST ). Introduction Past century has witnessed a tremendous increase in life expectancy of the human population, achi
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mCAP mg As small non coding RNAs microRNAs miRNAs can
2020-03-25
As small non-coding RNAs, microRNAs (miRNAs) can control gene expression at the post-transcriptional level by binding to the 3′-untranslated regions (3′-UTR) of messenger RNAs (mRNA) [15], [16]. miRNAs have been anticipated to regulate virtually all cellular mechanisms [17] and to play an important
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It was demonstrated that the
2020-03-25
It was demonstrated that the addition of 10-fold excess of α-synuclein without modifications (with respect to the molar concentration of tetrameric GAPDH) leads to partial inactivation of GAPDH after 1-h incubation by 20% (Fig. 4, curve 2) in focal adhesion kinase to insignificant decrease of the s
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