br Acknowledgements The study was

Acknowledgements The study was supported by grant no. 501-003-11043 from the Institute of Psychiatry and Neurology in Warsaw. The authors thank Mrs. Ala Biegaj for the excellent technical assistance.
Introduction In an effort to identify hIFN receptor molecules encoded by a gene(s) within human chromosome 21q, we applied the technique of DL-α-Difluoromethylornithine hydrochloride hydrate trapping to DNA obtained from three large YAC clones which span the hIFN receptor region (Chumakov et al., 1992). We isolated sequences as exons trapped in the splicing vector pMHC2 (Hamaguchi et al., 1992) and analyzed trapped exons by sequencing. Sequences that contained homology with known interferon receptors were pursued further. A 123-bp exon was identical to an exon in the human CRF2-4 gene (Lutfalla et al., 1993), and this trapped exon was used to isolate a full-length human CRF2-4 cDNA. Human CRF2-4 is a 325-aa, single membrane-spanning protein, of unknown function, that belongs to the interferon receptor family of proteins (class II cytokine receptor family). It may be a subunit of an interferon receptor complex, as the CRF2-4 gene (HGM designated CRFB4) on human chromosome 21 is between two interferon receptor genes. CRF2-4 is within 35 kb of the IFNAR1 gene (Lutfalla et al., 1993) and is within 1 kb of the IFNAR2 gene (Lutfalla et al., 1995). The human CRF2-4 cDNA was used as a probe to isolate a clone for mouse CRF2-4.
Experimental and discussion
Conclusions and discussion
Acknowledgements