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  • br Data This data article presents the protein

    2018-11-03


    Data This data article presents the protein level of HO-1 (Fig. 1) and CD200 (Fig. 2) secreted by BM-MSCs, WJ-MSCs and AT-MSCs in the absence (constitutive) or in the presence of activated T-cells (co-culture).
    Experimental design, materials and methods
    Acknowledgements We gratefully acknowledge the support received from the \"Fonds de la Recherche Scientifique (FNRS), and its Télévie program (Grant no. FC75708).
    Data The dataset of this article provides additional information to Ref. [1], in which we have characterized the increased susceptibility of C57BL/6 lipocalin 2 knockout (Lcn2KO) mice to colitis. Considering that the mouse genetic background can influence colitogenesis [i.e., immune responses of C57BL/6 and BALB/c mice are Th1 and Th2-biased, respectively [2–5], we herein investigated the susceptibility of BALB/c Lcn2KO mice to interleukin-10 receptor (IL-10R) neutralization-induced chronic colitis. The data presented here elucidate that the robust chronic colitis observed in αIL-10R-treated C57BL/6 Lcn2KO mice [1] can be recapitulated in BALB/c Lcn2KO mice. Specifically, splenomegaly, colomegaly, and elevated serum and colonic AZD-9291 markers were observed in αIL-10R-treated BALB/c Lcn2KO mice when compared to their respective WT control (Fig. 1A–E). Remarkably, BALB/c Lcn2KO mice exhibited rectal prolapse, a severe form of colitis, upon IL-10R neutralization (Fig. 1F). Histological analysis—extent of inflammatory cell infiltrate (ICI), epithelial hyperplasia, goblet cell loss, and distorted crypt structure (Fig. 1G and H)— further established that BALB/c Lcn2KO mice develop a severe chronic colitis, upon IL-10R neutralization, when compared to WT control.
    Experimental design, materials and methods
    Acknowledgements This work was supported by grant from the National Institutes of Health R01 (DK097865) to MV-K. VS is supported by CCFA׳s Research Fellowship Award (ID: 418507).
    Data
    Solar cell characterization The J–V characteristics of PCDTBT:PC70BM based devices, which were fabricated from a 10 and 15mg/ml photoactive layer dispersion, are shown in Figs. 3 and 4, respectively. The detailed device characteristics are shown in Supplementary information Table S1 and S2 by Kutsarov et al. [1]. It can be seen that the average characteristics of the cells are reduced, compared to the characteristics of the best performing cell. This is due to fabrication defects, as reported by Kutsarov et al. [1].
    Experimental design, materials and methods
    Sample and solar cell fabrication Prior to the deposition of the PCDTBT:PC70BM dispersion, the glass substrates were cleaned in an ultrasonic bath sequentially for 5min in Decon 90 detergent solution with deionized water (DI), DI, acetone, and methanol. Then, the substrates were blown dry with a nitrogen gun and treated with an oxygen plasma for 5min (100W, 15 sccm O2, Emitech K1050X plasma cleaner). The PCDTBT:PC70BM layers were deposited under ambient conditions on top of the glass substrate, at a substrate temperature of 50°C, 70°C, or 90°C. A flow rate of 100µl/min, coating speed of 18mm/s, and a screw gap of 200µm were used. After the deposition, the layers were annealed for 10min at 70°C to ensure the evaporation of any excess solvents. The complete devices comprised an inverted structure of glass-ITO/ZnO/PCDTBT:PC70BM/MoO3/Al and were fabricated according to the experimental details reported by Kutsarov et al. [1].
    Methods To characterize the PCDTBT:PC70BM layers absorption spectra, UV–visible (UV–vis) spectroscopy was used (Varian Cary 5000 UV–vis–NIR spectrophotometer). The spectra were recorded through a shadow mask with a circular opening (diameter of 5mm), which was placed at the center of the slot-die coated layer as reported by Kutsarov et al. [1]. The reported spectra represent the average of two spectra, measured for each sample in the wavelength range from 300nm to 900nm relative to a glass reference.