br Acknowledgements This study was supported by a Grant from

Acknowledgements This study was supported by a Grant from the Smoking Research Foundation (URL: http://www.srf.or.jp/english/index.html), Grant-in-Aid for Scientific Research on Innovative Areas 26118701, 26120701(Y.O.), and Grant-in-Aid for Young Scientists (A) 25713043 (Y.O.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We would like to thank Hiromi Matsubara and Tomoko Furukawa for helping us train rats. We also thank Katsuhiro Aikawa for technical support of surgery.
Data
Experimental design, materials and methods All specimens of C. andromeda were collected from the Nayband bay, in the North (27° 30´ S, 52° 35´ E) of Bushehr-Iran [1,2]. Nematocysts and tentacles were separated as described by Bloom et al. [3]. The mortality rate was measured within 24 hours, according to Wiltshire et al. [4]. For hematological parameters in vivo, eighteen male Wistar rats, (Weighing approximately 200±10g), were distributed randomly among three groups (two separated groups for test and the other group for control (n=6)). Animals in two tested groups were given two dilution series of 1/2 and 1/3 LD50 of jellyfish venom, respectively. The same volume of distilled water was injected into the other group as negative control. After 12h, the animals were anaesthetized by diethyl ether. Blood sample of each animal was collected by cardiac punctures, into both heparinized tubes for directly hematological tests, and without anticoagulant tubes for biochemical examinations. The performance evaluations of hematological parameters include RBC, WBC, Hb, MCV, MCH, MCHC and HCT were performed using the Sysmex XE-5000 hematology analyzer. All tests were done in triplicate and data were expressed as mean±SD. Statistical analysis was carried out using Kruskal–Wallis test. P<0.05 is considered statistically significant. The hemolysis experiment of crude venom was assessed using human pyruvate dehydrogenase kinase according to the method of Garnier et al. [5]. For chemical analysis of serum electrolytes, a portion of free anticoagulant blood samples, which previously described, were centrifuged at 600×g for 15 minutes, and the supernatant was separated as the serum. Serum albumin, urea, creatinine, uric acid, glucose, triglyceride, and haptoglobin were also measured.
Funding sources
Acknowledgments The authors gratefully acknowledge the help and financial support provided by Bushehr University of Medical Sciences and Health Services, Bushehr- Iran. Authors are thankful to Dr. P. Farzadinia, from Department of Anatomy, School of Medicine, in our university, for his scientific assistance.
Data The presented data include information on metabolites in blood and their concentration (Table 1), obtained by acetonitrile extraction (Fig. 1) and solid-state extraction (Fig. 2) methods.
Experimental design, materials and methods The experiment׳s planning, design and data processing correspond to the protocols given in Refs. [1–4].
Acknowledgements The work is supported by the Grant no. 14.575.21.0073, code RFMEFI57514×0073 of the Ministry of Education and Science of the Russian Federation.
Data We used iTRAQ labeling combined with LC-ESI-MS/MS analysis to identify proteins that were differentially expressed in gastric cancer tissues compared with adjacent normal tissues. The analysis identified 431 proteins that were differentially expressed, of which 224 and 207 proteins were expressed at increased or decreased levels, respectively, in gastric cancer tissues (≥1.2-fold change) (Tables 1 and 2). Our data showed that the differential expressed proteins included the proteins to the cellular components, molecular functions, and biological process in gastric cancer tissues. The validation studies showed that FABP1 was highly expressed in GC tissues (Fig. 1). FASN was found to be overexpressed in gastric cancer tissues (Fig. 2 and Table 3). An in-depth analysis of the data is presented in the associated research article [1].